The hospital in London, England that has been caring for him recently asked a judge to allow doctors to remove Charlie's life support without his parents' permission, the Daily Mail reported in March.
A statement from the Great Ormond Street Hospital, which was issued following the High Court ruling, said: "Our focus now is to work with Charlie's family to plan for the next stages in Charlie's care".
Hospital experts from Great Ormond Street Hospital (GOSH) argued in court that long-term life support should be withdrawn from Charlie because his quality of life was "so poor".
The doctor behind the experimental nucleoside therapy in America accepted that it was "very unlikely" that Charlie's condition would improve with the treatment. But Charlie's Mum and Dad objected, saying saying they believed he still had a chance if he underwent pioneering treatment in the United States.
The couple said they were devastated by the decision and were struggling to understand why the judge didn't give him the chance of treatment.
"What parents would not do the same?"
Pointing out the treatment trial would put Charlie through unnecessary pain, he said: "There is unanimity among the experts from whom I have heard that nucleoside therapy can not reverse structural brain damage".
Solicitor Laura Hobey-Hamsher said outside the court that the couple would have three weeks to launch a challenge.
Instead they recommended Charlie be taken off life support and transferred to palliative care.
Mr Gard cried "no", while other family members broke down in tears after hearing the judge's ruling. "The Great Ormond Street Hospital has made an application and it is my duty to rule on it, given that the parents and the hospital can not agree on the best way forward".
The parents had indicated previously that if they lost the case they might set up a charity for research into mitochondrial depletion syndrome with the money donated.
'Most importantly of all, I want to thank Charlie's parents for their fearless and dignified campaign on his behalf, but more than anything to pay tribute to their absolute dedication to their wonderful boy, from the day that he was born'. "Even if Charlie doesn't make it through this, I don't ever want another mum and their child to go through this".
A spokesperson for GoFundMetold Sky News they would talk with Charlie's parents over what will happen with the money raised for Charlie's treatment.
The therapy in the U.S. was "unknown territory " and there was unanimity from among experts he had heard from that structural brain damage could not be reversed, Francis said.
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